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货号 | 产品名称 | 规格 |
GOY-01K1660 | 肌动蛋白依赖染色质调控因子SMARCD3蛋白抗体 | 50ul |
GOY-01K1660 | 肌动蛋白依赖染色质调控因子SMARCD3蛋白抗体 | 100ul |
GOY-01K1660 | 肌动蛋白依赖染色质调控因子SMARCD3蛋白抗体 | 200ul |
英文名称: SMARCD3
中文名称: 肌动蛋白依赖染色质调控因子SMARCD3蛋白抗体
别 名；60 kDa BRG 1/Brm associated factor subunit C;
60 kDa BRG-1/Brm-associated factor subunit C; BAF60C; BRG1 associated factor
60C; BRG1-associated factor 60C; CRACD3; MGC111010; Rsc6p; Smarcd3;
SMRD3_HUMAN; SWI/SNF related matrix associated actin dependent regulator of
chromatin subfamily d member 3; SWI/SNF-related matrix-associated
actin-dependent regulator of chromatin subfamily D member 3.
研究领域；肿瘤 心血管 染色质和核信号 神经生物学 信号转导 转录调节因子 表观遗传学
抗体来源；Rabbit
克隆类型；Polyclonal
交叉反应；(predicted: Human, Mouse, Rat,
Chicken, Dog, Pig, Cow, Horse, Zebrafish, Sheep, )
产品应用；ELISA=1:5000-10000
IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in
other applications.
optimal
dilutions/concentrations should be determined by the end user.
理论分子量；55kDa
细胞定位；细胞核
性 状；Liquid
浓 度；1mg/ml
免 疫 原；KLH conjugated synthetic
peptide derived from human SMARCD3: 101-200/483
亚 型；IgG
纯化方法；affinity purified by Protein A
缓 冲 液；0.01M TBS(pH7.4) with 1% BSA,
0.03% Proclin300 and 50% Glycerol.
注意事项；This product as supplied is intended
for research use only, not for use in human, therapeutic or diagnostic
applications.
产品介绍 SMARCD3, is a member of the
SMARCD family and contains one SWIB domain. Two isoforms, isoform 1 and isoform
2 exist due to alternative splicing events. Both isoforms are expressed in
placenta, salivary gland, kidney, brain, trachea, uterus, prostate, testis,
thyroid, spleen and heart, while isoform 1 is also expressed in adipose tissue
and skeletal muscle. Localizing to the nucleus, SMARCD3 is a component of the
ATP-dependent chromatin remodeling complex SNF/SWI and is believed to play a
role in nucleosome remodeling. SMARCD3 also plays an important role in the
regulation of muscle development. In mice, the silencing of the gene en-coding
SMARCD3 leads to defects in heart morphogenesis. In addition, both isoforms of
SMARCD3 directly interact with and function as coactivators for several
transcription factors.
Plays a role in ATP dependent nucleosome remodeling bySMARCA4 containing complexes. Stimulates nuclear receptor mediatedtranscription. Belongs to the neural progenitors-specific chromatinremodeling complex (npBAF complex) and the neuron-specificchromatin remodeling complex (nBAF complex). During neuraldevelopment a switch from a stem/progenitor to a post-mitoticchromatin remodeling mechanism occurs as neurons exit the cellcycle and become committed to their adult state. The transitionfrom proliferating neural stem/progenitor cells to post-mitoticneurons requires a switch in subunit composition of the npBAF andnBAF complexes. As neural progenitors exit mitosis anddifferentiate into neurons, npBAF complexes which containACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologousalternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunitsin neuron-specific complexes (nBAF). The npBAF complex is essentialfor the self-renewal/proliferative capacity of the multipotentneural stem cells. The nBAF complex along with CREST plays a roleregulating the activity of genes essential for dendrite growth (Bysimilarity).
实验流程：
（1）特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而，抗体可通过与病毒或毒素的特异性结合，直接发挥中和病毒的作用。
（2）活补体：IgM、IgG1、IgG2和IgG3可通过经典途径激活补体，凝聚的IgA、IgG4和IgE可通过替代途径激活补体。
（3）结合细胞：不同类别的免疫球蛋白，可结合不同种的细胞，参与免疫应答。
（4）可通过胎盘及粘膜：免疫球蛋白G（IgG）能通过胎盘进入胎儿血流中，使胎儿形成自然被动
免疫。免疫球蛋白A（IgA）可通过消化道及呼吸道粘膜，是粘膜局部抗感染免疫的主要因素。
（5）具有抗原性：抗体分子是一种蛋白质，也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子，各具有不同的抗原性。
（6）抗体对理化因子的抵抗力与一般球蛋白相同：不耐热，60～70℃即被破坏。各种酶及能使蛋白质凝固变性的物质，均能破坏抗体的作用。抗体可被中性盐类沉淀。在生产上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白，再经透析法将其纯化。
抗体的制备过程：
1.免疫原：普通的大分子蛋白，通过分子克隆构建载体并在大肠杆菌中进行诱导表达获得重组蛋白，纯化鉴定后可直接作为免疫原；小分子蛋白或化合物等分子量小，需要偶联载体对该分子进行改造才能使其成为具有免疫原性的抗原，常见偶联载体如BSA、OVA、HAS等。
2. 免疫动物：常用于制备抗血清的动物有豚鼠、家兔、鸡、大小鼠等，大量生产时需要用到狗、绵羊、山羊等。
3.免疫血清的收集：一般家兔、绵羊、山羊可采用静动脉采血，家兔、豚鼠、大鼠、鸡可采用心脏采血，家兔、山羊、绵羊可采用静脉采血。
4. 免疫血清的纯化与鉴定：得到的抗血清需要进一步的纯化，利用偶联了抗原的亲和柱进行层析，具有高效，特异性强，纯度高的特定。接着要鉴定纯化蛋白的含量、相对分子的质量、纯度以及特异性。
5肌动蛋白依赖染色质调控因子SMARCD3蛋白抗体.免疫血清的保存：抗体一般比较稳定，在-80℃ ～-20 ℃可以保存约5年而不会影响效价，而真空干燥保存时间可以更久。保存前需经除菌并添加防腐剂。
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